Activities

Activities

Services, equipment reservation and pricing

ARPEGE : The different stages of your project with us!

For services, our platform offer to customers a wide range of cellular assays in order to characterize pharmacological targets and/or validate hits obtained by screening. The activity of ARPEGE facility is therefore positioned upstream and downstream of high throughput screening. Thanks to a liquid handling robot, our facility can also carry out small screenings (100 – 1000 molecules) for exploratory assays.

Define the project feasibility, technical requirements

Study of your project

The ARPEGE team studies your project and its technical feasibility

Conduct experiments

The team carries out the experiments

Our team takes charge of your project and carries out the experiments studied and planned upstream

Analysis and interpretation of results

The team analyzes the results of the experiments

The ARPEGE platform analyzes all the results and uses its expertise to support your project

Analyse and interpret results

Analyse and interpret results,

Our team ensures the monitoring and evolution of your project

To go further…

Accompaniement

The ARPEGE platform supports you with training, the development of innovative techniques and the development of specific tests

ARPEGE : Free access services

As part of its open access services, the platform ensures:

Staff presence from 9am to 12pm and from 1pm to 5pm, and undertakes to inform customers in the event of staff absence

Online publication of its prices (device time & consumables), validated by the CNRS. The prices applied for billing purposes are those in use at the time the equipment is used, and are therefore subject to change. The customer will be notified of any change in pricing

Optimum equipment performance through periodic checks and regular maintenance

Making inspection results available to customers on request

Guiding customers towards the techniques best suited to their needs

Training customers in the use of machines and providing them with the necessary tutorials

Training customers in experimental data processing on request

Guarantee of data obtained in compliance with the protocol for use of the equipment

Application of health and safety instructions

Provision of consumables and chemicals listed on the platform’s online site (pricing)

Intervention within 24 hours in the event of a breakdown, and undertakes to keep customers informed of the progress made

Informing customers of equipment non-compliance

ARPEGE : Services

The platform staff will support you along your project by providing advice, training on specific devices and in some cases, by performing required experiments for the completion of your project.

Technical specifications for platform services:

  • transient expression system: HEK293
  • transient transfection: lipofectamin, electroporation
  • assays formats: 96 – 384 wells

Possibility to perform assays on customers cell lines. Contact the platform staff for more information.

Available assays:

BRET

BRET: activation of G proteins (Gi1, i2, i3, oA, oB, z, q, 13), recruitment of GRK2, 3, 5, recruitment of arrestins 1, 2, cAMP sensor (CAMYEL)

Bioluminescence

cAMP GloSensor (Promega)

Fluorescence

Calcium sensors (Fluo4-AM, Cal520), potassium sensor (FluxOR)

TR-FRET, HTRF

TR-FRET, HTRF : binding, internalisation, second messengers (IP1, cAMP, etc.), kinase phosphorylation (ERK1/2, etc.)

A number of GPCRs have been tested and validated on signalling pathways by the platform staff like MOR, b2AR, PAR1, mGluR…

For more information you can contact us.

ARPEGE : Formation

The platform offers theoretical and practical training:

This training is aimed at customers of the platform to enable them to use the protocols and machines necessary for experiments.

The workshops are interactive in nature and therefore allow everyone to intervene and ask for additional information or explanations. For any information regarding the organization of these workshops, please contact the platform team directly.

This work is reserved for Master 2 students.

ARPEGE : Equipment reservation

An online reservation system has been set up for the use of the platform’s machines.

Possible access to the ARPEGE reservation system.

ARPEGE : Pricing

Commencing the 1st September 2011, the ARPEGE platform has defined new prices:

 

Depending on services and experimentation type, pricing includes equipment maintenance, ongoing costs of the platform, and some products and consumables.

Contact the platform staff for more details.

Note: In the context of providing a service for private laboratories, pricing also includes the use of machines and the time spent by the platform staff on the project.

ARPEGE : Resources

The platform offers its customers different techniques based on the principles of fluorescence and resonance energy transfer (BRET, FRET, TR-FRET) for the study of molecular interactions.

In order to better understand these techniques and their uses, the platform staff has written a document bringing together the fundamental notions of fluorescence and resonance energy transfer. The principle of these techniques is illustrated through examples of protein-protein interactions.

Questions?

For any request regarding the ARPEGE platform, our activity and our services, do not hesitate to contact us