Equipements & Technologies
Equipements & Technologies
Equipements & Technologies de la plateforme ARPEGE
Accueil / Equipement & Technologies / Mithras LB940
Mithras LB940
Responsable technique : Claire VOL
Applications :
- interactions protéines-protéines,
- production de seconds messagers
| MODULE/FUNCTION | DESCRIPTION |
|---|---|
| Detection Modes | BRET |
| Measurement Modes | Top and bottom reading, Endpoint and Kinetic measurements, Sequential Multi Excitation measurements, Sequential Multi Emission measurements, Simultaneous Dual Emission measurements, Real-time ratiometric measurements, Well Scanning |
| Microplate Formats | Up to 384-well plates |
| Light Sources | Halogen lamp |
| Detectors | Low-noise photomultiplier with single photon counting technology |
| Excitation filters | 355 nm, 485 nm |
| Emission filters | 410 nm, 460 nm, 485 nm, 515 nm, 530 nm, 535 nm. |
| Sensitivity | Fluorescence <20 fmol FITC, Luminescence <20 amol ATP |
| Crosstalk | Low crosstalk due to crosstalk reduction methods : 5×10-6 |
| Temperature control | +5°C above room temperature to 45°C |
| Reagent Injection | Up to 4 injectors, JET injection technology, Variable volumes : 10 – 100 µL, Injection into microplates with up to 384 wells. |
| Shaking | 3 modes : linear, orbital, double-orbital, Variable amplitude and speed |
| Operation modes | |
| Integral measurement | 0.1 – 600 secondes |
| Kinetics measurement | Total length up to 24h |
| Repeated measurement | Total length up to 24h |
| Plate repeats | Up to 999 |
| Scanning | Up to 100 single data points |
| Delay | Delay |
