Technical manager: Claire VOL
Applications:
– calcium release (Fluo4)
Others applications: contact the platform staff.
Technical specifications:
| MODULE/FUNCTION | DESCRIPTION |
|---|---|
| Detection method | Fluorescence |
| Plate formats | 96 wells |
| Light source | Xenon Flash Lamp (1 joule/flash) |
| Detectors | 2 photomultiplier tubes |
| Exitation | 485 nm |
| Emission | 538 nm |
| Flex reading | Fluorescence + fluidics |
| Kinetic reading | Fluorescence + fluidics |
| Fluidics (96-Well Plates Only) | 8-channel |
| Max. volume | 200 μL |
| Precision @ 50 μL | 2% CV |
| Precision @ 5 μL | 8% CV |
| Dispense max. rate | 208 μL/sec. |
| Sensitivity | < 5 pM fluorescein in 96 wells, < 20 pM in 384 wells |
| Typical Read Times With 3 flashes/well in fluorescence mode | 96 wells : 17 seconds |
| Temp. control* | 2°C above ambient to 45°C |
| Temp. uniformity* | < 1°C at 37°C set point |
| Temp. accuracy* | ±1°C at 37°C set point |
| *Temperature is regulated in the Read Chamber | |
| Shaker time | 0 to 999 seconds |