Technical manager: Isabelle Brabet
Applications:
– Protein-protein interaction,
– ligand-receptor interaction,
– Second messengers production…
Others applications: contact the platform staff.
Technical specifications:
| MODULE/FUNCTION | DESCRIPTION |
|---|---|
| Detection Modes | Fluorescence, TR-FRET/HTRF®, BRET |
| Microplate Formats | 6- to 384-well plates |
| Light Sources | High energy xenon flash lamp |
| Optics | Fused Silica Lenses |
| Detectors | Fluorescence : low dark current photomultiplier tube (PMT) |
| Excitation | 320 nm, 340 nm, 485 nm, 535 nm, 590 nm, 610 nm. |
| Emission | 485 nm, 520 nm, 590 nm, 620 nm, 640 nm, 665 nm, 670 nm. |
| Sensitivity | Fluorescence top reading : 10 amol / well Fluorescein (Greiner® 384-well low volume black plate ; 10 μl) Fluorescence bottom reading : 0.4 fmol / well Fluorescein (Greiner 96-well SensoPlate™ ; 200 μl), TRF : 0.5 amol / well Europium (Greiner 384-well low volume white plate ; 10 μl, 100 flashes). |
| Read Times | 1 flash / well fluorescence intensity top 384 well plates : kinetic cycle : < 19 sec. plate in/out : < 35 sec. |
| Temperature control | from 4°C above ambient up to 42°C |
| Injectors | 3 different syringe sizes : 500 μl, 1000 μl, 2500 μl. |
| Shaking | Linear and orbital shaking, Amplitude selectable from 0.5 to 6 mm. |