Technical manager: Damien Maurel
Applications:
– Protein-protein interaction,
– ligand-receptor interaction,
– Second messengers production…
Others applications: contact the platform staff.
Technical specifications:
| MODULE/FUNCTION | DESCRIPTION |
|---|---|
| Detection Modes | Fluorescence, TR-FRET/HTRF®, AlphaScreen® |
| Measurement Modes | Top and bottom reading, Endpoint and Kinetic measurements, Sequential Multi Excitation measurements, Sequential Multi Emission measurements, Simultaneous Dual Emission measurements, Real-time ratiometric measurements, Well Scanning. |
| Microplate Formats | Up to 384-well plates, user-definable |
| Light Sources | High energy xenon flash lamp, Nitrogen Laser (337 nm), Solid state Laser for AlphaScreen®. |
| Detectors | Four photomultiplier tubes, optimized for different detection modes |
| Z-Adjustment | Automatic focal height adjustment (0.1 mm resolution) |
| Excitation | 337 nm, 485 nm, 640 nm, 680 nm |
| Emission | 490 nm, 520 nm, 550 nm, 570 nm, 620 nm, 665 nm, 680 nm. |
| Decay | TR-FRET decay measurement in real time with 2 µsec resolution |
| Sensitivity | TRF 25 fmol/L europium (0.5 amol/well) (384), 100 fmol/L europium (0.8 amol/well) (1536). HTRF® Reader Control Kit (Eu) after 18h (384), Delta F > 1100 % (High Calibrator), Delta F > 25 % (Low Calibrator). AlphaScreen® < 100 amol / well (384) |
| Read Times | 1 flash 14 s (384), 27 s (1536) 10 flashes 38 s (384), 1 min 52 s (1536) 50 flashes 1 min 29 s (384), 5 min 18 s (1536) |
| Incubation | +5°C above ambient up to 45°C |
| Reagent Injection | Up to 2 built-in reagent injectors Injection at measurement position (6 to 384-well) Individual injection volumes for each well (3 to 350 µL) Variable injection speed up to 420 µL / s Up to four injection events per well Reagent back flushing |
| Shaking | Linear, orbital, and double-orbital with user-definable time and speed |