Responsable technique : Claire VOL
Applications :
– interactions protéines-protéines,
– productions de seconds messagers…
Autres applications : voir le responsable technique.
Spécification technique pour l’application :
| MODULE/FUNCTION | DESCRIPTION |
|---|---|
| Detection Modes | BRET |
| Measurement Modes | Top and bottom reading, Endpoint and Kinetic measurements, Sequential Multi Excitation measurements, Sequential Multi Emission measurements, Simultaneous Dual Emission measurements, Real-time ratiometric measurements, Well Scanning. |
| Microplate Formats | Up to 384-well plates |
| Light Sources | Halogen lamp |
| Detectors | Low-noise photomultiplier with single photon counting technology |
| Excitation filters | 355 nm, 405 nm, 485 nm. |
| Emission filters | 410 nm (BRET2), 460 nm, 480 nm (BRET1), 515 nm (BRET2), 535 nm, 540 nm (BRET1), 560 nm. |
| Sensitivity | Fluorescence <20 fmol FITC, Luminescence <20 amol ATP. |
| Crosstalk | Low crosstalk due to crosstalk reduction methods : 5x10-6 |
| Temperature control | +5°C above room temperature to 45°C |
| Reagent Injection | Up to 4 injectors, JET injection technology, Variable volumes : 10 - 100 µL, Injection into microplates with up to 384 wells. |
| Shaking | 3 modes : linear, orbital, double-orbital, Variable amplitude and speed |
| Operation modes | |
| Integral measurement | 0.1 - 600 secondes |
| Kinetics measurement | Total length up to 24h |
| Repeated measurement | Total length up to 24h |
| Plate repeats | Up to 999 |
| Scanning | Up to 100 single data points |
| Delay | Up to 600 second |