FLEXstation 3


Responsable technique : Claire VOL

Applications :

– signaux calciques intracellulaires (Fluo3, Fluo4)

Autres applications : voir le responsable technique.

Spécification technique pour l’application :

Detection methodFluorescence
Plate formats96 wells
Light sourceXenon Flash Lamp (1 joule/flash)
Detectors2 photomultiplier tubes
Exitation485 nm
Emission538 nm
Flex readingFluorescence + fluidics
Kinetic readingFluorescence + fluidics
Fluidics (96-Well Plates Only)8-channel
Max. volume200 μL
Precision @ 50 μL2% CV
Precision @ 5 μL8% CV
Dispense max. rate208 μL/sec.
Sensitivity< 5 pM fluorescein in 96 wells, < 20 pM in 384 wells
Typical Read Times
With 3 flashes/well in fluorescence mode
96 wells : 17 seconds
Temp. control*2°C above ambient to 45°C
Temp. uniformity*< 1°C at 37°C set point
Temp. accuracy*±1°C at 37°C set point
*Temperature is regulated in the Read Chamber
Shaker time0 to 999 seconds