For services, our platform offer to customers a wide range of cellular assays in order to characterize pharmacological targets and/or validate hits obtained by screening. The activity of ARPEGE facility is therefore positioned upstream and downstream of high throughput screening. Thanks to a liquid handling robot, our facility can also carry out small screenings (100 – 1000 molecules) for exploratory assays.
The platform staff assists customers to:
– define the project feasibility, technical requirements,
– conduct experiments,
– analyse and interpret results,
– follow-up the project.
An accompanying staff member is also available for:
– training,
– development of innovative techniques,
– development of specific assays.
Services offered by the platform:
FULL SERVICES
The platform staff will support you along your project by providing advice, training on specific devices and in some cases, by performing required experiments for the completion of your project.
Technical specifications for platform services:
– transient expression system: HEK293
– transient transfection: lipofectamin, electroporation
– assays formats: 96 – 384 wells
Possibility to perform assays on customers cell lines. Contact the platform staff for more information.
Available assays:
Luminescence (fluorescence, bioluminescence, FRET, BRET, HTRF, TR-FRET)
- BRET: activation of G proteins (Gi1, i2, i3, oA, oB, z, q, 13), recruitment of GRK2, 3, 5, recruitment of arrestins 1, 2, cAMP sensor (CAMYEL)
- Bioluminescence: cAMP GloSensor (Promega)
- Fluorescence: calcium sensors (Fluo4-AM, Cal520), potassium sensor (FluxOR)
- TR-FRET, HTRF *: binding, internalisation, second messengers (IP1, cAMP, etc.), kinase phosphorylation (ERK1/2, etc.).
* Commercial kits: https://fr.cisbio.eu/dd/by-universe/gpcr-platform
A number of GPCRs have been tested and validated on signalling pathways by the platform staff like MOR, b2AR, PAR1, mGluR… For more information contact the platform staff.
« OPEN ACCESS »
The platform makes the equipment available in an open access area. You can use this equipment to perform your own experiments and manage your data once trained by the technicians or the manager in charge of the machine.
PARTNERSHIP AND COLLABORATION
It is possible to implement a partnership and/or a collaboration contract in order to carry out your research projects.
Service offer is made available to academic laboratories, biotech as well as pharma and other industrial companies.