Spark 20M

Technical manager: Damien Maurel

Applications:

– Protein-protein interaction,
– ligand-receptor interaction,
– Second messengers production…

Others applications: contact the platform staff.

Technical specifications:

Module/FunctionDescription
Detections modeAbsorbance, fluorescence, TR-FRET/HTRF®
Plate compatibilityUp to 1536-well plates
Light SourceFlash lamp, 20 Watt, 100 Hz
Detectorsphoto-multiplier tube (PMT)
Monochromator230 - 900 nm with variable bandwidth: 5, 7.5, 10, 15, 20, 25, 30, 50 nm
Excitation filters340/20, 360/35, 485/20, 520/10, 620/10
Emission filters465/35, 535/25, 544/25, 595/35, 620/20, 665/8
Mirrors5 mirror positions:
50% mirror, 510 Dichroic, 560 Dichroic, 630 Dichroic, user-definable Dichroic (410 Dichroic or 430 Dichroic)
TemperatureRegulation is possible between 20 °C and 42 °C
ShakingLinear, orbital and double orbital